Summary
Type: SPE
Application #: 304780
Catalog#: 730952 - Chromab. Columns HR-XC, 3 ml, 200 mg
Detection:
Conditions: Column conditioning: 5 mL methanol, then 5 mL water
(Do not let run the column dry!)
Sample aspiration: The prepared sample is passed through the column by vacuum or pressure
Washing: With 2 mL 0.1 N HCl impurities are removed
Drying: With nitrogen or air
Elution:
Fraction A (acidic and neutral analytes) is eluted with 2.5 mL methanol;
Fraction B (basic analytes) with 5.0 mL methanol / NH3 (90:10, v/v)
Evaporation and reconstitution with 1 mL of mobile phase from subsequent HPLC
Authors: SPE department
Source: Macherey-Nagel